3/30/2023 0 Comments Western blot ladderThe pen is optimized for detection using the C-DiGit Blot Scanner or the Odyssey Fc Imaging System, and is also suitable for use with film or other imaging systems. Western blot primary and secondary antibodies employed for the detection of. The WesternSure Pen is used to annotate visible protein ladders (such as Chameleon Vue, P/N 928-50000) prior to chemiluminescent Western blot detection. Not intended for human or animal diagnostic or therapeutic uses. The Spectra pre-stained multicolor protein ladder (Thermo Fisher, Waltham. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Stable for up to 2 weeks at 25☌long term storage, store at -20☌ Note : Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to a nitrocellulose membrane. Apply more for thicker (> 1.5 mm) or larger gel.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.1.5~2.5 μl per well for general Western transferring.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis conditions are listed below: Guide for Molecular Weight Estimation (kDa) Do not heat, dilute, add reducing agent before loading. The ladder is supplied in gel loading buffer and is ready to use. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. 01SDS-PAGE 02Western Blotting 03CBB staining 04Silver staining. At Cell Signaling Technology (CST) we understand that western blotting experiments are time consuming and that their success has a critical impact on your research progress. GangNam-STAIN Prestained Protein Ladder is a protein marker that can identify the. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500550 nm channel). The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site.
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